Project
Part:BBa_K2103001
Designed by: Maggie Chen Group: iGEM16_CCA_SanDiego (2016-10-12)
NADPH P450 Reductase (Homo Sapiens)
Isolation of the NPR reductase from the original plasmid caused differing restriction sites, bridged by oligonucleotides with common overhangs to both sites and a poly-A tail in between the overhangs. Subsequent ligation into the pSB1c3 plasmid gives the Biobrick preffix, followed by the NADPH Biobrick, followed by HindIII restriction site, and lastly the Biobrick suffix.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 659
Illegal NgoMIV site found at 778
Illegal NgoMIV site found at 1413 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 106
Illegal BsaI.rc site found at 421
Illegal BsaI.rc site found at 1404
Illegal SapI.rc site found at 785
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Categories
Parameters
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