Project

Part:BBa_K2103001

Designed by: Maggie Chen   Group: iGEM16_CCA_SanDiego   (2016-10-12)


NADPH P450 Reductase (Homo Sapiens)

Isolation of the NPR reductase from the original plasmid caused differing restriction sites, bridged by oligonucleotides with common overhangs to both sites and a poly-A tail in between the overhangs. Subsequent ligation into the pSB1c3 plasmid gives the Biobrick preffix, followed by the NADPH Biobrick, followed by HindIII restriction site, and lastly the Biobrick suffix.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 659
    Illegal NgoMIV site found at 778
    Illegal NgoMIV site found at 1413
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 106
    Illegal BsaI.rc site found at 421
    Illegal BsaI.rc site found at 1404
    Illegal SapI.rc site found at 785


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